| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tan, Sayjel E (16)
|
MRQNS |
24.54 |
26.21 |
1.67 |
| 2 |
Brulinski, Natalia E (17)
|
MRQNS |
26.69 |
26.51 |
-0.18 |
| 3 |
Chang, Isabella M (15)
|
MRQNS |
26.18 |
26.85 |
0.67 |
| 4 |
Ha, Kara (16)
|
MRLIFE |
26.43 |
27.35 |
0.92 |
| 5 |
Smith, Jamila (16)
|
MRNFS |
27.67 |
27.95 |
0.28 |
| 5 |
Bieganowski, Maya A (15)
|
MRQNS |
26.11 |
27.95 |
1.84 |
| 5 |
Loftus, Ava E (15)
|
MRQNS |
26.42 |
27.95 |
1.53 |
| 8 |
Regan, Jaclyn R (16)
|
MRQNS |
29.03 |
28.27 |
-0.76 |
| 9 |
Bouzarouata, Salma K (15)
|
MRQNS |
28.67 |
28.80 |
0.13 |
| 10 |
Zhang, Erica A (15)
|
MRQNS |
29.04 |
28.81 |
-0.23 |
| 11 |
Wynntar-Oo, Isabella A (15)
|
MRQNS |
30.59 |
29.78 |
-0.81 |
| 12 |
Lewis, Teagan M (15)
|
MRSSC |
32.53 |
31.87 |
-0.66 |
| 13 |
LoBello, Allison (16)
|
MRLIFE |
39.33 |
36.60 |
-2.73 |